Effects of a Standardized Phenolic-Enriched Maple Syrup Extract on β-Amyloid Aggregation, Neuroinflammation in Microglial and Neuronal Cells, and β-Amyloid Induced Neurotoxicity in Caenorhabditis elegans
dc.contributor.author | Ma, Hang | |
dc.contributor.author | DaSilva, Nicholas A. | |
dc.contributor.author | Liu, Weixi | |
dc.contributor.author | Nahar, Pragati P. | |
dc.contributor.author | Wei, Zhengxi | |
dc.contributor.author | Liu, Yongqiang | |
dc.contributor.author | Pham, Priscilla T. | |
dc.contributor.author | Crews, Rebecca | |
dc.contributor.author | Vattem, Dhiraj | |
dc.contributor.author | Slitt, Angela L. | |
dc.contributor.author | Shaikh, Zahir A. | |
dc.contributor.author | Seeram, Navindra | |
dc.date.accessioned | 2020-04-13T21:19:50Z | |
dc.date.available | 2020-04-13T21:19:50Z | |
dc.date.issued | 2016-11 | |
dc.description.abstract | Published data supports the neuroprotective effects of several phenolic-containing natural products, including certain fruit, berries, spices, nuts, green tea, and olive oil. However, limited data are available for phenolic-containing plant-derived natural sweeteners including maple syrup. Herein, we investigated the neuroprotective effects of a chemically standardized phenolic-enriched maple syrup extract (MSX) using a combination of biophysical, in vitro, and in vivo studies. Based on biophysical data (Thioflavin T assay, transmission electron microscopy, circular dichroism, dynamic light scattering, and zeta potential), MSX reduced amyloid β1-42 peptide (Aβ1-42) fibrillation in a concentration-dependent manner (50-500 μg/mL) with similar effects as the neuroprotective polyphenol, resveratrol, at its highest test concentration (63.5 % at 500 μg/mL vs. 77.3 % at 50 μg/mL, respectively). MSX (100 μg/mL) decreased H2O2-induced oxidative stress (16.1 % decrease in ROS levels compared to control), and down-regulated the production of lipopolysaccharide (LPS)-stimulated inflammatory markers (22.1, 19.9, 74.8, and 87.6 % decrease in NOS, IL-6, PGE2, and TNFα levels, respectively, compared to control) in murine BV-2 microglial cells. Moreover, in a non-contact co-culture cell model, differentiated human SH-SY5Y neuronal cells were exposed to conditioned media from BV-2 cells treated with MSX (100 μg/mL) and LPS or LPS alone. MSX-BV-2 media increased SH-SY5Y cell viability by 13.8 % compared to media collected from LPS-BV-2 treated cells. Also, MSX (10 μg/mL) showed protective effects against Aβ1-42 induced neurotoxicity and paralysis in Caenorhabditis elegans in vivo. These data support the potential neuroprotective effects of MSX warranting further studies on this natural product. | |
dc.description.department | Family and Consumer Sciences | |
dc.description.version | This is the accepted manuscript version of an article published in Neurochemical Research. | |
dc.format | Text | |
dc.format.extent | 23 pages | |
dc.format.medium | 1 file (.pdf) | |
dc.identifier.citation | Ma, H., DaSilva, N. A., Liu, W., Nahar, P. P., Wei, Z., Liu, Y., Pham, P. T., Crews, R., Vattem, D. A., Slitt, A. L., Shaikh, Z. A., & Seeram, N. P. (2016). Effects of a standardized phenolic-enriched maple syrup extract on β-amyloid aggregation, neuroinflammation in microglial and neuronal cells, and β-amyloid induced neurotoxicity in Caenorhabditis elegans. Neurochemical Research, 41(11), pp. 2836-2847. | |
dc.identifier.doi | https://doi.org/10.1007/s11064-016-1998-6 | |
dc.identifier.issn | 0364-3190 | |
dc.identifier.uri | https://hdl.handle.net/10877/9593 | |
dc.language.iso | en | |
dc.publisher | Springer | |
dc.source | Neurochemical Research, 2016, Vol. 41, No. 11, pp. 2836-2847. | |
dc.subject | Amyloid 42 | |
dc.subject | caenorhabditis elegans | |
dc.subject | maple syrup phenolics | |
dc.subject | neuroinflammation | |
dc.subject | neuroprotective | |
dc.subject | Alzheimer's disease | |
dc.title | Effects of a Standardized Phenolic-Enriched Maple Syrup Extract on β-Amyloid Aggregation, Neuroinflammation in Microglial and Neuronal Cells, and β-Amyloid Induced Neurotoxicity in Caenorhabditis elegans | |
dc.type | Article |