Effect of Bacteriophage 92 Infection in Combination With Teicoplanin on Mixed-Community Methicillin-Resistant Staphylococcus Aureus and Staphylococcus Epidermidis Biofilms

Date

2013-08

Authors

Riggs, Jon Maston, Jr.

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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is an aggressive pathogen, which is classified as a healthcare-associated infection(HAI). HAis are a significant problem for the U.S. healthcare industry, with approximately 1.8 million patients affected each year. The emergence of antibiotic resistance among bacteria has resulted in the use of bacteriophage infection in combination with antibiotics to treat infection. Staphylococcus epidermidis is a commensal found in mixed-community biofilms with S. aureus. To date there have been no reports on the effect of phage and antibiotic on S. aureus and S. epidermidis mixed-community biofilms. This study reports the effect of the antibiotic teicoplanin in combination with Staphylococcus bacteriophage type 92 on the survival rates of mixed-community S. aureus and S. epidermidis 48 h biofilms. Teicoplanin is a glycopeptide antibiotic with a similar spectrum of activity to vancomycin and used in the treatment of MRSA infections. The results demonstrate phage infection in combination with teicoplanin reduced S. aureus monoculture biofilm better (89% kill) than phage alone (40% kill) or teicoplanin alone (73% kill). Teicoplanin reduced S. epidermidis monoculture biofilm better (87% kill) than phage alone (24% kill) or teicoplanin in combination with phage (55% kill). In mixed-community biofilms, phage alone was more effective against S. aureus (96% kill) than teicoplanin (55% kill) or a combination of phage and teicoplanin (88%). The combination of teicoplanin and bacteriophage was more effective against S. aureus monocultures than either teicoplanin or phage alone, and increased susceptibility of S. aureus to phage infection was observed when present in mixed-community with S. epidermidis. However, S. epidermidis acquired teicoplanin resistance when present in mixed-community biofilms with S. aureus.

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Keywords

bacteriophages, antibiotics, staphylococcus aureus, methicillin resistance, staphylococcus, biofilms

Citation

Riggs, J.M. (2013). Effect of bacteriophage 92 infection in combination with teicoplanin on mixed-community methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis biofilms (Unpublished thesis). Texas State University-San Marcos, San Marcos, Texas.

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