The Effects of Neuromuscular Electrical Stimulation on Anabolic Signaling in Older Adults

dc.contributor.advisorMettler, Joni A.
dc.contributor.authorGoldenstein, Samantha
dc.contributor.committeeMemberKipp, Lindsay
dc.contributor.committeeMemberMcAllister, Matt
dc.description.abstractNeuromuscular electrical stimulation (NMES) generates involuntary muscle contraction and may be a safe and effective alternative for voluntary resistance training. However, further research needs to be done to fully understand the effects of NMES on muscle strength, self-efficacy for activities of daily living (ADL’s), and on anabolic signaling. <b>PURPOSE:</b> The purpose of this study was to investigate the effects of a 12 session, 4-week neuromuscular electrical stimulation (NMES) intervention in healthy, older adults. The outcomes investigated were anabolic signaling, strength, and self-efficacy of daily activities. <b>METHODS:</b> Participants (n = 11; NMES = 8, Sham = 3) consisted of healthy, older adults (mean age: 71.7 ± 7.2 years). Participants performed maximal voluntary contractions (MVC) using the quadriceps on an isokinetic dynamometer, a 5RSTS test, and completed a survey about their self-efficacy for ADL’s pre-post-Intervention. Day 1 and Day 12 of the intervention consisted of a muscle biopsy pre-NMES, 30 min post-NMES, and 120 min post-NMES. The participants were randomly placed in a treatment group (NMES) or Sham group. The participants were treated with exact same protocol except the Sham group did not receive stimulation. The NMES was administered 3 times a week for 4-weeks (12 sessions) at 60 Hz for 40 minutes on each leg. <b>RESULTS:</b> Phosphorylated S6K1( p = 0.020) and phosphorylated mTOR (p = 0.009) had a significant main effect for time (S6K1 Day 1: Pre-NMES 0.65 ± 0.17, Post-30min 0.98 ± 0.17, Post-120min 1.01 ± 0.19; Post-Intervention: Day 12: PreNMES 0.63 ± 0.17, Post-30min 1.25 ± 0.17, Post-120min 0.89 ± 0.21) (mTOR Day 1: Pre-NMES 0.46 ± 0.13, Post-30min 1.01 ± 0.13, Post-120min 0.92 ± 0.14; Day 12: PreNMES 0.49 ± 0.13, Post-30min 0.74 ± 0.14, Post-120min 0.48 ± 0.16) and the post hoc revealed Post-30 min was significantly upregulated when compared to Pre-NMES for both proteins (S6K1 p = 0.017, mTOR p = 0.007). There was no main effect or interaction for phosphorylated 4E-BP1, MVC, or 5RSTS. The intervention-by-group interaction for ADL Self-efficacy had a medium effect size (η2 = 0.197). <b>CONCLUSION:</b> The findings of this study suggest that a 4-week session of NMES upregulates signaling proteins of the mTORC1 pathway (p-mTOR and p-S6K1) 30 minutes after stimulation. Even though there was no significant difference in MVC or 5RST, there was a medium effect size for self-efficacy ADLs for this preliminary data set. Therefore, further research with more subjects is warranted in order to better understand the effects of this 4-week NMES intervention in older adults.
dc.description.departmentHealth and Human Performance
dc.format.extent98 pages
dc.format.medium1 file (.pdf)
dc.identifier.citationGoldenstein, S. (2019). <i>The effects of neuromuscular electrical stimulation on anabolic signaling in older adults</i> (Unpublished thesis). Texas State University, San Marcos, Texas.
dc.subjectNeuromuscular electrical stimulation
dc.subjectOlder adults
dc.subjectAnabolic signaling
dc.titleThe Effects of Neuromuscular Electrical Stimulation on Anabolic Signaling in Older Adults
dc.typeThesis and Human Performance Science State University of Science


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