Dasari, RameshLa Clair, JamesKornienko, Alexander2019-10-182019-10-182017-09Dasari, R., La Clair, J. J., & Kornienko, A. (2017). Irreversible protein labeling by Paal-Knorr conjugation. ChemBioChem, 18(18), pp. 1792–1796.1439-4227https://hdl.handle.net/10877/8714The application of new chemical reactions in a biological context has advanced bioconjugation methods for both fundamental research and commercial arenas. Recent adaptations of reactions such as Huisgen 1,3-dipolar or Diels-Alder cycloadditions have enabled the labeling of specific residues in biomolecules by the attachment of molecules carrying azides, alkynes, or strained alkenes. Although these are fundamental tools, there is a need for the discovery of reactions that can label native proteins. We report herein the adaptation of the Paal-Knorr reaction to label lysine residues in proteins via pyrrole linkages.Text15 pages1 file (.pdf)enbiocompatible reactionsbioconjugationclick chemistryprotein labelingPaal-Knorr reactionChemistry and BiochemistryArticlehttps://doi.org/10.1002/cbic.201700210