Alternative Splicing of Arabidopsis IBR5 Pre-mRNA Generates Two IBR5 Isoforms with Distinct and Overlapping Functions

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dc.contributor.authorJayaweera, Thilanka
dc.contributor.authorSiriwardana, Chamindika
dc.contributor.authorDharmasiri, Sunethra
dc.contributor.authorQuint, Marcel
dc.contributor.authorGray, William M.
dc.contributor.authorDharmasiri, Nihal
dc.date.accessioned2021-08-09T18:45:42Z
dc.date.available2021-08-09T18:45:42Z
dc.date.issued2014-08
dc.description.abstractThe INDOLE-3-BUTYRIC ACID RESPONSE5 (IBR5) gene encodes a dual specificity phosphatase that regulates plant auxin responses. IBR5 has been predicted to generate two transcripts through alternative splicing, but alternative splicing of IBR5 has not been confirmed experimentally. The previously characterized ibr5-1 null mutant exhibits many auxin related defects such as auxin insensitive primary root growth, defective vascular development, short stature and reduced lateral root development. However, whether all these defects are caused by the lack of phosphatase activity is not clear. Here we describe two new auxin insensitive IBR5 alleles, ibr5-4, a catalytic site mutant, and ibr5-5, a splice site mutant. Characterization of these new mutants indicates that IBR5 is post-transcriptionally regulated to generate two transcripts, AT2G04550.1 and AT2G04550.3, and consequently two IBR5 isoforms, IBR5.1 and IBR5.3. The IBR5.1 isoform exhibits phosphatase catalytic activity that is required for both proper degradation of Aux/IAA proteins and auxin-induced gene expression. These two processes are independently regulated by IBR5.1. Comparison of new mutant alleles with ibr5-1 indicates that all three mutant alleles share many phenotypes. However, each allele also confers distinct defects implicating IBR5 isoform specific functions. Some of these functions are independent of IBR5.1 catalytic activity. Additionally, analysis of these new mutant alleles suggests that IBR5 may link ABP1 and SCF TIR1/AFBs auxin signaling pathways.
dc.description.departmentBiology
dc.formatText
dc.format.extent15 pages
dc.format.medium1 file (.pdf)
dc.identifier.citationJayaweera, T., Siriwardana, C., Dharmasiri, S., Quint, M., Gray, W. M., & Dharmasiri, N. (2014). Alternative splicing of Arabidopsis IBR5 pre-mRNA generates two IBR5 isoforms with distinct and overlapping functions. PLoS One, 9(8), e102301.
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0102301
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/10877/14252
dc.language.isoen
dc.publisherPublic Library of Science
dc.rights.holder© 2014 Jayaweera et al.
dc.rights.licenseThis work is licensed under a Creative Commons Attribution 4.0 International License.
dc.sourcePLoS One, 2014, Vol. 9, No. 8, Article e102301.
dc.subjectauxins
dc.subjectseedlings
dc.subjectphosphatases
dc.subjectArabidopsis thaliana
dc.subjectgene expression
dc.subjectlateral roots
dc.subjectalternative splicing
dc.subjectphenotypes
dc.subjectBiology
dc.titleAlternative Splicing of Arabidopsis IBR5 Pre-mRNA Generates Two IBR5 Isoforms with Distinct and Overlapping Functions
dc.typeArticle

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