Synthesis, Characterization, and In Vitro Evaluation of Near-Infrared Absorbing Conductive Polymer Nanoparticles as Agents for Photothermal Ablation of Breast Cancer Cells

dc.contributor.advisorBetancourt, Tania
dc.contributor.advisorIrvin, Jennifer A.
dc.contributor.authorCantu, Travis
dc.contributor.committeeMemberTunnell, James
dc.contributor.committeeMemberWeigum, Shannon
dc.contributor.committeeMemberBurns, John
dc.date.accessioned2017-04-10T17:51:50Z
dc.date.available2017-04-10T17:51:50Z
dc.date.issued2015-12
dc.description.abstractIn recent years, photothermal therapy (PTT) has emerged as a viable alternative for cancer treatment. Much effort has been devoted to finding various types of PTT agents with excellent heat generation, photothermal efficiency, biocompatibility, and biodegradability. In this work, polymeric nanoparticles (NPs) composed of poly(1,4-bis(3,4-ethylenedioxythienyl)-2,5-dialkoxybenzenes) (PBEDOT-B(OR)2) and poly(3,4-ethylenedioxythiophene) (PEDOT) were synthesized using microemulsion polymerization. The NPs were characterized using dynamic light scattering, UV-Vis-NIR spectroscopy, and electron microscopy. The microemulsion polymerization yielded sub–100 nm NPs and the colloidal suspensions exhibited a strong absorbance in the near infrared region. The photothermal transduction and efficiency of these materials were determined and compared to those of commonly used PTT agents. When irradiated with NIR light, the suspensions showed a temperature change of ca. 30 °C with a photothermal efficiency of ca. 35%. In vitro cytocompatibility studies were also performed on the conductive polymeric NPs in an effort to determine the concentration limits that could be used without causing toxicity to cells. Cytocompatibility studies for the colloid suspensions were conducted at 24 h and exposure times, and the NPs were found to be non-toxic at a dose of 50 μg/mL. Photothermal in vitro studies also demonstrated that cell death can be achieved after 5 min of irradiation at concentrations as low as 0.5 μg/mL. These results suggest that these materials could be good candidates for use as photothermal therapy (PTT) agents.
dc.description.departmentMaterials Science, Engineering, and Commercialization
dc.formatText
dc.format.extent143 pages
dc.format.medium1 file (.pdf)
dc.identifier.citationCantu, T. (2015). Synthesis, characterization, and in vitro evaluation of near-infrared absorbing conductive polymer nanoparticles as agents for photothermal ablation of breast cancer cells (Unpublished dissertation). Texas State University, San Marcos, Texas.
dc.identifier.urihttps://hdl.handle.net/10877/6548
dc.language.isoen
dc.subjectPhotothermal therapy
dc.subjectConductive polymers
dc.subjectNanoparticles
dc.subjectBreast cancer
dc.subjectIn vitro studies
dc.titleSynthesis, Characterization, and In Vitro Evaluation of Near-Infrared Absorbing Conductive Polymer Nanoparticles as Agents for Photothermal Ablation of Breast Cancer Cells
dc.typeDissertation
thesis.degree.departmentMaterials Science, Engineering, and Commercialization Program
thesis.degree.disciplineChemistry and Biochemistry
thesis.degree.grantorTexas State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy

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