Identification and Characterization of Bacterial Isolates from Spring Lake, Texas
| dc.contributor.advisor | McLean, Robert J. C. | |
| dc.contributor.author | Merchant, Mubina M. | |
| dc.date.accessioned | 2020-07-23T17:31:22Z | |
| dc.date.available | 2020-07-23T17:31:22Z | |
| dc.date.issued | 2005-05 | |
| dc.description.abstract | Eight bacterial isolates were retrieved from a biofilm during a homoserine lactone recruitment experiment in Spring Lake, Texas. On the basis of morphology, cellular fatty acid analysis, and 16S-23S intergenic transcribed spacer region sequencing, seven of the eight isolates were identified as Chromobacterium violaceum. A polyphasic approach to taxonomy was used to characterize the eighth, potentially novel isolate, A62-14B. The isolate was identified as a gamma proteobacterium, sharing 95-96% 16S rRNA gene sequence similarity to the genera Rheinheimera and Alishewanella. The 16S rRNA gene sequence data allowed for a novel species designation for isolate A62-14B, but did not resolve genus level relationships. Fatty acid analysis supported an association at the genus level of isolate A62-14B with Rheinheimera. Further physiological and morphological characterization via biochemical tests and electron microscopy revealed that the isolate shared a variety of key phenotypic traits with both related genera. It was concluded that isolate A62-14B represented a novel species, but a generic designation could not be assigned. The characterization of this organism revealed the short comings currently in bacterial taxonomy, including issues with a lack of taxon sampling, inability to compare and fully characterize unculturable organisms, and the lack of stringent control over experimental conditions for the comparison of organisms. | |
| dc.description.department | Biology | |
| dc.format | Text | |
| dc.format.extent | 77 pages | |
| dc.format.medium | 1 file (.pdf) | |
| dc.identifier.citation | Merchant, M. M. (2005). Identification and characterization of bacterial isolates from Spring Lake, Texas (Unpublished thesis). Texas State University-San Marcos, San Marcos, Texas. | |
| dc.identifier.uri | https://hdl.handle.net/10877/12151 | |
| dc.language.iso | en | |
| dc.subject | bacteria | |
| dc.subject | Spring Lake | |
| dc.subject | isolates | |
| dc.title | Identification and Characterization of Bacterial Isolates from Spring Lake, Texas | |
| dc.type | Thesis | |
| thesis.degree.department | Biology | |
| thesis.degree.grantor | Texas State University-San Marcos | |
| thesis.degree.level | Masters | |
| thesis.degree.name | Master of Science |
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