Characterization of Supra-Concentrate of Human Bone Marrow Stem Cells for the Treatment of Spine and Musculoskeletal Disorders
The use of autogenous stem cells is one of the pillars of regenerative medicine. The past one and a half decades have witnessed the discovery and characterization of a new type of adult stem cells named Very Small Embryonic-like stem (VSEL) cells. These cells exhibit pluripotency, they are localized in many tissues, including bone marrow and mobilized peripheral blood, which makes them easily accessible to the clinician. However, there are two major impediments to implementing their use in a clinical setting. First, current protocols that use blood or marrow as the source of therapeutic concentrates, such as platelet rich plasma (PRP) or bone marrow concentrate (BMAC), respectively, have not been characterized in terms of human VSEL (hVSEL) cell content. And second, there are no protocols to produce a concentrate of autologous hVSEL cells that can be implemented and in a clinical setting. STUDY OBJECTIVES 1. To characterize the current centrifugation method used in most clinics for nucleated cell concentration in terms of hVSEL cells yield, and 2. To develop a method for the enrichment of hVSEL cells derived from human bone marrow that is amenable to full implementation in a clinical setting.
regenerative medicine, VSEL, hVSEL, hMSC, stem cells, multipotency, pluripotency
Pedrozo, H., & Dumitrescu, M. (2023). Characterization of supra-concentrate of human bone marrow stem cells for the treatment of spine and musculoskeletal disorders. Poster presented at the Health Scholar Showcase, Translational Health Research Center, San Marcos, Texas.