Evidence for Transcapillary Movement of Exogenous Superoxide Dismutase and Catalase Into Dog Myocardium
Date
1989-05
Authors
Chudej, Laurie L.
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Abstract
The production of oxygen radicals has been shown to play a role in reperfusion injury following a short period of ischemia. Superoxide dismutase (SOD) and catalase (CAT) were infused into the coronary circulation prior to and during 15 minutes of ischemia to determine if the enzymes could reduce reperfusion injury caused by the production of oxygen free radicals and determine where the SOD and CAT exert their effect. As measured by sonomicrometry, reperfusion injury was reduced by SOD and CAT in these preparations within one minute of reperfusion. Reperfusion was followed by intracoronary fixation. Using transmission electron microscopy, electron dense material was observed in vesicles of capillary endothelia, between capillaries and myocyte, and in vesicles within myocytes. This material was only observed in hearts treated with SOD and/or CAT suggesting concentration and transport of CAT and SOD by vesicles across the capillary endothelium and into the myocyte. Similar electron dense material was also detected between the capillary endothelia and myocytes in hearts infused with SOD and CAT and not subjected to ischemia or reperfusion . Vesicular transport rate across capillaries was assessed by computer assisted planimetry. An increase in transport was evident in reperfused myocardium which had been infused with SOD and CAT. These results indicate transcapillary movement of SOD and/or CAT by endocytosis and exocytosis across the endothelium and uptake of SOD and/or CAT by myocytes.
Description
Keywords
dogs, cardiovascular system, catalase, myocardium
Citation
Chudej, L.L. (1989). Evidence for transcapillary movement of exogenous superoxide dismutase and catalase into dog myocardium (Unpublished thesis). Southwest Texas State University, San Marcos, Texas.