The Proline/Glycine-Rich Region of the Biofilm Adhesion Protein Aap Forms an Extended Stalk that Resists Compaction

dc.contributor.authorYarawsky, Alexander
dc.contributor.authorEnglish, Lance R.
dc.contributor.authorWhitten, Steven T.
dc.contributor.authorHerr, Andrew
dc.date.accessioned2020-04-13T17:19:36Z
dc.date.available2020-04-13T17:19:36Z
dc.date.issued2017-01
dc.description.abstractStaphylococcus epidermidis is one of the primary bacterial species responsible for healthcare-associated infections. The most significant virulence factor for S. epidermidis is its ability to form a biofilm, which renders the bacteria highly resistant to host immune responses and antibiotic action. Intercellular adhesion within the biofilm is mediated by the accumulation-associated protein (Aap), a cell wall-anchored protein that self-assembles in a zinc-dependent manner. The C-terminal portion of Aap contains a 135-aa-long, proline/glycine-rich region (PGR) that has not yet been characterized. The region contains a set of 18 nearly identical AEPGKP repeats. Analysis of the PGR using biophysical techniques demonstrated the region is a highly extended, intrinsically disordered polypeptide with unusually high polyproline type II helix propensity. In contrast to many intrinsically disordered polypeptides, there was a minimal temperature dependence of the global conformational state of PGR in solution as measured by analytical ultracentrifugation and dynamic light scattering. Furthermore, PGR was resistant to conformational collapse or α-helix formation upon the addition of the osmolyte trimethylamine N-oxide or the cosolvent 2,2,2-trifluoroethanol. Collectively, these results suggest PGR functions as a resilient, extended stalk that projects the rest of Aap outward from the bacterial cell wall, promoting intercellular adhesion between cells in the biofilm. This work sheds light on regions of low complexity often found near the attachment point of bacterial cell wall-anchored proteins.
dc.description.departmentChemistry and Biochemistry
dc.description.versionThis is the accepted manuscript version of an article published in the Journal of Molecular Biology.
dc.formatText
dc.format.extent35 pages
dc.format.medium1 file (.pdf)
dc.identifier.citationYarawsky, A. E., English, L. R., Whitten, S. T., & Herr, A. B. (2017). The proline/glycine-rich region of the biofilm adhesion protein Aap forms an extended stalk that resists compaction. Journal of Molecular Biology, 429(2), pp. 261–279.
dc.identifier.doihttps://doi.org/10.1016/j.jmb.2016.11.017
dc.identifier.issn0022-2836
dc.identifier.urihttps://hdl.handle.net/10877/9590
dc.language.isoen
dc.publisherElsevier
dc.sourceJournal of Molecular Biology, 2017, Vol. 429, No. 2, pp. 261–279.
dc.subjectanalytical ultracentrifugation
dc.subjectcell wall-anchored protein
dc.subjectcircular dichroism
dc.subjectintrinsically disordered protein
dc.subjectstaphylococcus epidermidis
dc.subjectChemistry and Biochemistry
dc.titleThe Proline/Glycine-Rich Region of the Biofilm Adhesion Protein Aap Forms an Extended Stalk that Resists Compaction
dc.typeArticle

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