The Effects of TMPyP4 on G-Quadruplex Forming Oligonucleotides
The ability of guanine-rich DNA to adopt various folded forms is well-established and these G-quadruplex structures are likely involved in telomere maintenance. The folded structures are stabilized in solution by binding of cations such as Na+ and certain small molecule ligands. One such ligand, TMPyP4, has previously been demonstrated to interfere with telomeric DNA processing by binding to G-quadruplexes in the DNA. However, for the G-quadruplex forming oligonucleotide, Tel22 (based on the human telomeric DNA sequence) TMPyP4 significantly decreases the stability of the folded structure in the presence of varying levels of Na+ ion. This destabilization is unusual and implications for further studies are discussed.
G-quadruplex, ligands, TMPyP4, molecular interactions, Honors College
Gonzalez, D. C. (2022). The effects of TMPyP4 on G-quadruplex forming oligonucleotides (Unpublished thesis). Texas State University, San Marcos, Texas.