Exogenous Superoxide Dismutase and Catalase Prevent Reperfusion Injury in Rat Myocardium and may be Transported From Capillaries Into Myocytes




Christodoulides, Nicolaos Joseph

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The effects of infusing superoxide dismutase (SOD)and catalase (CAT) into the coronary circulation were investigated in rat hearts (isolated, working) prior to and during a 15 minute episode of regional ischemia followed by 30 minutes reperfusion. Aortic output (AO), left ventricular pressure (L VP) and dP/dT of L VP were recorded. Compared to sham treated hearts, SOD and CAT significantly improved function during reperfusion, but had no effect during the pre-ischemic or the ischemic period. Cryotome sections of isolated, angendorf[ perfused (with or without SOD or CAT) rat hearts were exposed to rabbit antibody prepared against the exogenous SOD and CAT. Bound antibody was detected by the indirect-fluorescent antibody test. The interior of myocytes from rat hearts exposed to SOD and CAT bound antibodies prepared against these enzymes; whereas, myocytes from rat hearts not exposed to exogenous SOD and CAT only bound the CAT antibodies. This indicates the anti-SOD we prepared is specific for exogenous SOD, and also suggests exogenous SOD is transported efficiently from the coronary circulation into the cytoplasm of myocytes.



catalase, myocardium, rats, cardiovascular system


Christodoulides, N.J. (1989). Exogenous superoxide dismutase and catalase prevent reperfusion injury in rat myocardium and may be transported from capillaries into myocytes (Unpublished thesis). Southwest Texas State University, San Marcos, Texas.


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